Journal: Frontiers in Immunology

Article Title: Integrated multi-omics and machine learning identify core mechanobiology-associated immune genes in pulmonary arterial hypertension

doi: 10.3389/fimmu.2026.1867114

Figure Lengend Snippet: Primer sequences.

Article Snippet: The primary antibodies utilized in this study included: TLR7 (17232-1-AP), CD86 (26903-1-AP), CD83 (27873-1-AP), IL-6 (21865-1-AP), and α-Tubulin (80762-1-RR), all purchased from Proteintech, Rosemont, USA.

Techniques:

Journal: Frontiers in Immunology

Article Title: Integrated multi-omics and machine learning identify core mechanobiology-associated immune genes in pulmonary arterial hypertension

doi: 10.3389/fimmu.2026.1867114

Figure Lengend Snippet: Transparent candidate prioritization and diagnostic value of the experimentally examined four-gene panel. (A) Candidate funnel from mechanical source genes to PAH-MRG overlap, variable PAH-MRG genes, machine-learning-stable candidates and the four-gene panel. (B) LASSO coefficient paths and random-forest importance. (C) Shapley contribution of CD83, CD86, IL6 and TLR7 in the four-gene logistic model. (D) Cross-layer direction of the four genes in lung bulk, PBMC and single-cell data. (E) Single-cell expression across cell types. (F) Four-gene disease-state score ROC and calibration. (G) Per-gene ROC curves in lung and PBMC. The ML component is presented as candidate prioritization rather than a deployable diagnostic model.

Article Snippet: The primary antibodies utilized in this study included: TLR7 (17232-1-AP), CD86 (26903-1-AP), CD83 (27873-1-AP), IL-6 (21865-1-AP), and α-Tubulin (80762-1-RR), all purchased from Proteintech, Rosemont, USA.

Techniques: Diagnostic Assay, Single Cell, Expressing

Journal: Frontiers in Immunology

Article Title: Integrated multi-omics and machine learning identify core mechanobiology-associated immune genes in pulmonary arterial hypertension

doi: 10.3389/fimmu.2026.1867114

Figure Lengend Snippet: Single-cell localization of the four-gene panel. (A) UMAP visualization of annotated lung cell types split by control and IPAH status. (B) Cellular composition comparison between control and IPAH. (C) Canonical marker dot plot supporting cell-type annotation. (D) Feature plots and violin plots showing CD83, CD86, IL6 and TLR7 expression across disease groups. CD83 and CD86 localize mainly to myeloid/macrophage and antigen-presenting compartments, whereas IL6 shows structural-cell enrichment and TLR7 is sparsely detected.

Article Snippet: The primary antibodies utilized in this study included: TLR7 (17232-1-AP), CD86 (26903-1-AP), CD83 (27873-1-AP), IL-6 (21865-1-AP), and α-Tubulin (80762-1-RR), all purchased from Proteintech, Rosemont, USA.

Techniques: Single Cell, Control, Comparison, Marker, Expressing, Gene Expression

Journal: Frontiers in Immunology

Article Title: Integrated multi-omics and machine learning identify core mechanobiology-associated immune genes in pulmonary arterial hypertension

doi: 10.3389/fimmu.2026.1867114

Figure Lengend Snippet: Establishment of MCT-induced pulmonary hypertension (PH) rat model recapitulating PAH pathological features. (A) RVSP and (B) RV/(LV+S) were detected in MCT-induced pulmonary hypertension (PH) rats and control rats (n=6/group). (C) Representative H&E staining images of lung tissues from the two groups. Black asterisks mark the pulmonary artery lumen. Scale bar = 100 μm (n=6/group). (D) Representative Western blot bands and quantitative analysis of CD86, TLR7, CD83, IL-6, and GAPDH in lung tissues of MCT-induced PH rats and control rats. Data represent mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001 compared to control, as analyzed by unpaired t test or Mann-Whitney test as appropriate.

Article Snippet: The primary antibodies utilized in this study included: TLR7 (17232-1-AP), CD86 (26903-1-AP), CD83 (27873-1-AP), IL-6 (21865-1-AP), and α-Tubulin (80762-1-RR), all purchased from Proteintech, Rosemont, USA.

Techniques: Control, Staining, Western Blot, Analysis, MANN-WHITNEY

Journal: Frontiers in Immunology

Article Title: Integrated multi-omics and machine learning identify core mechanobiology-associated immune genes in pulmonary arterial hypertension

doi: 10.3389/fimmu.2026.1867114

Figure Lengend Snippet: Biomarker validation in clinical IPAH peripheral blood mononuclear cell specimens. (A) Expression levels of CD86, TLR7, CD83 and IL-6 in clinical peripheral blood mononuclear cell (PBMC) samples. (B) ROC curve analysis of the diagnostic efficacy of CD86, TLR7, CD83 and IL-6 for IPAH. Data represent mean ± SEM. *** P < 0.001 compared to control, as analyzed by unpaired t test.

Article Snippet: The primary antibodies utilized in this study included: TLR7 (17232-1-AP), CD86 (26903-1-AP), CD83 (27873-1-AP), IL-6 (21865-1-AP), and α-Tubulin (80762-1-RR), all purchased from Proteintech, Rosemont, USA.

Techniques: Biomarker Discovery, Expressing, Analysis, Diagnostic Assay, Control